Upgrading Pippin Instrument Software 1. Insert the USB drive with the upgrade folder into the USB port on the front panel of the BluePippin instrument. 2. From the Main Tab, press the "INFO" button. This will launch the information window. 3. From the Information Window, press "SOFTWARE UPGRADE", press"OK" at the warning prompt.The Pippin platform is typically used to construct genomic libraries for next-gen sequencing. The BluePippin is an updated version of Sage's Pippin Prep system, to which a pulsed-field power ...Disease Susceptibility Ranking of Apples. Name. Fire blight. Apple Scab. Powdery Mildew. Cedar Apple Rust. Leaf Spots ***. Ace Delicious. Moderately Resistant 4.Cassettes are disposable and pre-cast with agarose and running buffer. Samples are not optically detected.Blotting kits include all the components required to transfer protein, DNA, or RNA samples onto membranes for staining. The materials are grouped specifically for the method and the tissue specimen being implemented, allowing the entire procedure to be completed more rapidly. Using the ...In total, 48 unique barcode adapters were used in initial ligation, and sub-pools of these 48 barcodes were size selected using a 1.5% agarose gel cassette and target size selection of “narrow 450 bp” on a Blue Pippin electrophoresis unit …Upgrading the Pippin Instrument Software. 71. 21 Preventative Maintenance - Rinsing Electrodes. 73. Electrode Rinse Procedure. 73. 22 Warranty and Service. 74. ... 3 Blue-Pippin Installation Procedure. 5. Packaging and Components. 5. Instrument and Monitor Dimensions. 5. Installation. 6. Rear Panel of the Bluepippin. 7.Merry And Pippin. His eyes are so blue ahh!! Save. Save. More like this. a close up of a person with blue eyes and curly hair wearing a black coat. This much ...Blue Apron Coupon. Teachers take $160 on their Blue Apron order. Since 1957, GQ has inspired men to look sharper and live smarter with its unparalleled coverage of style, culture, and beyond. From ...The Blue Pippin also does your size selection, so you don't burn out pores sequencing lots of little stuff. This should help yield too. Then it's all down to the library prep, and @gringer's answer has some good tips on that. Share. Improve this answer. FollowThe resulting SMRTcell templates were size-selected to 15–50 kb by Blue Pippin electrophoresis (Sage Sciences) and sequenced on a PacBio RS II instrument using P6-C4 sequencing chemistry. Data ..."The Pippin Prep gave very tight size distributions whereas those from the Labchip XT were much broader. … The distribution of recovered fragment sizes was much more reproducible with the Pippin Prep." Such tight sizing can lead to lower yield, the authors note, adding that "this can partially be overcome by setting the instrument to ...Blue: 6: 1,170: Flush Tether: FLT: Purple: 1: 200: 3rd party materials Consumables. Agencourt AMPure XP beads; NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (E7180S) Alternatively, you can purchase NEB reagents individually: NEBNext FFPE Repair Mix (M6630)Internal price (Cornell and Cornell affiliates) External price. Fragment analysis, ready-to-load, per 96-well plate. $42.50. $69.70. LIZ500 and HiDi Formamide addition, per 96-well plate. $40.00. $65.60. ROX500 and HiDi Formamide addition, per 96-well plate. For the BluePippin, we have developed a protocol for this purpose, and named the cassette file "0.75% Greater Than - Marker S1". This requires our 0.75% dye-free gel cassettes kit for lower ranges (BLF7510). With this protocol, users enter a 4 hour run time, and enter a 50,000 bp value into the "BP End" field using the "Range ...Finally, large fragments (>10 kb) were enriched with Blue Pippin device (Sage Science, Inc., Beverly, MA, USA) for sequencing. A total of 15 single-molecule real-time (SMRT) cells were processed, 7 with Sequel and 8 with RS II instruments (PacBio), to generate a total of 67.32 Gb PacBio data. For Oxford Nanopore sequencing, ∼5 μg of genomic ...Finally, we pooled 1 μl of each dual-indexed library, creating four separate pools (i.e. fungal and bacterial libraries for leaf and rhizosphere samples). We used Blue Pippin (Sage Science Inc., Beverly, MA, USA) to select fragments that were between 150 and 600 bp, and then combined the four pools at equimolar concentrations.The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. ...Safety Data Sheet In accordance with Regulation (EC) No 1272/2008 (REACH) Trade name: BluePippin™ Agarose Gel Cassettes Product No: BDQ3010, BEF2010, BDF2010, BDF1510, PAC20KB, PAC30KB,Blue Pippin and E-gel size selection systems; Agilent Bioanalyzer; Eppendorf epMotion M5073 liquid handling system; GTF Staff are available to answer questions regarding the use of technologies offered by the GTF and provide consultation on experimental design.Libraries were prepared with Illumina TruSeq Stranded mRNA kit starting from 600 ng of total RNA, and samples were fragmented and amplified for 15 PCR cycles. Libraries were size selected with Blue Pippin (Sage Science) using 1.5% gel cassettes and 350-550 bp regions isolated.Description DNA Gel Cassettes Specifications Downloads Size Select Up To 24 Samples In One Run PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. Sage Science一直没有停止创新的脚步，推出了PippinHT高通量型号，一次可处理多至24个样本，相当于5台Pippin PrepTM在同时运行。 特点： 和Pippin的其它型号一样，PippinHT也是基于预制胶技术，在一块预制胶上可以上样12个样本，通量更高且成本更低。The Pippin platform has since established itself as a key platform for the construction of high-quality libraries for next-gen sequencing. In 2014 Sage Science released a new electrophoresis platform, the SageELF for whole sample fractionation of DNA or proteins, and the PippinHT , a high-throughput version of the BluePippin.Genomics Facility Depending on the results from the DNA sizing with the Fragment Analyzer, you may want to remove unwanted products from your library preparation. The Blue Pippin is used to select DNA of certain sizes and effectively remove unwanted fragment sizes. Individual libraries were normalized to 10 nM and pooled. The library pool was purified with the QIAquick PCR Purification Kit (Qiagen AB, Sweden) according to instructions. Automated size selection was performed using the Blue Pippin (Sage Science, Beverly, MA, USA), with a range of 135 to 175 bp to select the ~152 bp fragments.Individuals were then pooled, followed by a size selection step with the Blue Pippin (Sage Science, Beverly, MA) where all loci between 415 and 515 bp were retained. A final PCR step using Phusion Taq polymerase (New England Biolabs) with the following thermocycler conditions was conducted to amplify all loci and attach a 6bp index unique to ...The High Pass Plus™ gel cassette is the newest addition to the Pippin Family. As the name suggests, it is dedicated to our BluePippin "High-Pass" DNA size selection which has been a go-to method for increasing the read lengths for long-read sequencing. ... (100% up from standard Blue Pippin cassettes). We've also increased the size of ...In brief, genomic DNA was extracted from peripheral blood leukocytes using a QIAamp DNA Blood Mini Kit (Qiagen). DNA purity, integrity, and concentration were assayed using agarose gel electrophoresis and Qubit. Large DNA fragments (> 8 to 10 kb) were recovered using a Blue Pippin system (Sage Science, Beverly, MA, USA).Find the nearest stores. Search Address. Use current location. Seiko Prospex remains a standard for high-intensity timekeeping excellence. From the introduction of its first diver’s watch in 1965, Seiko has been a leader in undersea precision and durability, spearheading innovations in both technology and design.Scottie's latest target is none other than Michael Jordan. Pippen's beef is centered on the "The Last Dance," the 10-part ESPN produced documentary that captivated the country in 2020 ...BluePippin Operations Manual 460013 Rev K 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is anSize select the DNA with Blue Pippin, using High Pass Plus gel cassette and 0.75% Dye Free for 1–10 kb External Standards S1 according to manufacturer’s protocol. Further, 10 μg of DNA is required for each lane, and typically the amplified sample is enough for two lanes. Adjust the cutoff length depending on the fragment size distribution.The desired product was confirmed by running agarose gel (0.8%) electrophoresis and purified by using Blue pippin (Sage Science) (Supplementary Fig. 1b). Single-molecule distance measurement using ...The Sage Science portfolio includes products to perform DNA or cDNA sizing and protein fractionation. pippin.png. DNA Size Selection System. Pippin Prep ...We purified the input of 3.2 µg purified SMRTbell library to load into the Blue Pippin 0.75% Agarose Cassette using cassette definition 0.75% DF Marer S1 3 to 10 kb Improved Recovery for the run protocol. We collected fragments greater than 7 kb from the cassette elution well and purified and concentrated the size-selected SMRTbell library ...BluePippin Operations Manual 460013 Rev D 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is anBlue Pippin Size Selection : $285.00 : $356.25 : NanoString Services (reagents not included) nCounter MAX full-service (price per cartridge (12 samples)) $560.50 : $700.63 : GeoMx DSP (price per slide) $750.50 : $938.13 : Single-Cell Solutions (price per hour, reagents not included) 10X bench self-service : $95.00 : $118.75The Blue Pippin is utilized for DNA size selection (up to 50kb) for NGS. Desired target sizes or ranges of sizes are entered in software and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination....There are three main categories of colors: primary, secondary, and tertiary colors. The three primary colors are red, blue, and yellow. These colors occur naturally in nature and are on the light spectrum, so no color combine to make blue.The BluePippin (jpg) has the capabilities of the Pippin Prep, plus pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer with no possibility of cross contamination in the cassette.This was followed by phenol-chloroform purification and size selection to a minimum of 30 kb using a Blue Pippin. The resulting product was concentrated using ampure beads before library preparation was performed using a Rapid Barcoding Sequencing Kit (SQK-RBK001) modified through exclusion of LLB beads.selection with either AMPure PB beads (red) or the BluePippin system (blue). Since the genomic DNA were partially degraded, SMRTbell Libraries were constructed without additional fragmentation. Long continuous read lengths are essential for applications such as de novo assembly and Isoform Sequencing (Iso-Seq).The Blue Pippin is a powerful automated tool to collect DNA fragments up to 50 kb or size select proteins. It facilitates library construction for the most popular Next Generation Sequencing …To generate Nanopore long reads, ~15 µg of genomic DNA was size-selected (>20 Kb) with a Blue Pippin System (Sage Science). According to 1D Genomic DNA by Ligation protocol (Oxford Nanopore Technologies, ONT), ONT SQK-LSK108 library preparation kit was used to construct PCR-free libraries. Nextomics (Nextomic Biosciences Technologies ...Horace Pippin (American, 1888-1946). Self-Portrait, 1941. Oil on Canvas Board. 19 x 8 x 9" 14 x 11 inches (35.56 x 27.94 cm). Collection Albright-Knox Art Gallery, Buffalo, New York Room of Contemporary Art Fund, 1942 RCA1942:2. In the self-portrait, Pippin sits before a nondescript light blue background with his legs casually spread, wearing ...BLU0001 The BluePippin System includes instrument, monitor, keyboard, and mouse. Dimensions are 7"H X 11"W X 21"D, and weight is 15 lbs. Requires 100-240 VAC.Briefly, genomic DNA was sheared to ~8 KB using g-tubes (Covaris, Woburn, MA, USA) and size-selected with the Blue Pippin system (Sage Science, Beverly, MA, USA), collecting fragments that ranged ...Molecular characterization is a key step in the risk assessment of genetically modified organisms (GMOs) for regulatory approval. Herein, we describe a method for analyzing copy number, insertion loci, and flanking sequences through whole-genome sequencing (WGS) and bioinformatics. Comprehensive molecular characterization of G2-6 transgenic rice was performed using this pipeline. The results ...31 thg 8, 2019 ... 182 lbs Round Of 16 - Kaleb Horn, Blue Ridge vs Evan Pippin, North Alabama Wrestling Club.blue light for visualization. Do not use ethidium bromide and/or UV light since they can induce DNA damage. The SYBR stains can be easily removed from nucleic acids during the gel-extraction process, e.g., using Qiagen® gel-extraction kits. 2) Purity of your DNA sample: DNA purity can be determined by using the NanoDrop® instrument or otherThe BluePippin is a powerful automated DNA size selection system to collect DNA fragments up to 50 kb or size select proteins. It facilitates library construction for the most popular NGS platforms and is a fast and highly accurate and reproducible option over traditional gel extractions. The BluePippin works by pulsed-field electrophoresis to ...The repaired/modified DNA was used for V3 adapter Ligation. The adapter ligated library was treated with Enzyme mix for Nuclease treatment to remove damaged or non-intact SMRTbell templates. The purified library was then size selected using two-step size selection with Blue Pippin (Sage Science) generating 9-13 kb and > 15 kb fractions.In the first of the six runs, involving 13 samples, we used a Blue Pippin instrument to size-select DNA amplicons before sequencing. This was done to separate microbiome from host mitochondrial 16S rRNA gene sequences. This method proved untenable due to Blue Pippin performance inconsistencies and we did not apply …First-strand cDNA was synthesized using a SMARTer PCR cDNA Synthesis Kit (Clontech, CA, United States). After a few rounds of PCR amplification, the amplified cDNA was divided into different size fractions to prevent preferential small template sequencing using a Blue Pippin (Sage Science, MA, United States).Nick Pippin (16) QB. ... Official Home of Millikin Big Blue Athletics. Header logo. Menu. Sports. Men's ...Like the Blue Pippin, the PippinHT has pulsed field capabilities, thus enabling the size selection of DNA fragments intended for use in both short-read (including the Illumina iSeq 100, MiniSeq, MiSeq, NextSeq, HiSeq, NovaSeq, Ion Torrent PYRO 454) and long-read (Oxford Nanopore and PACBIO) NGS workflows. Proprietary Electro-elution TechnologyDIVE INTO INDIGO. Trippin' Blue is a denim research trip that inspires through creative innovation and new production horizons. We know how to generate your brand differentiation …Both approaches produced similar total yields of high-quality reads (roughly 3.5 gigabases, as shown by the point at which each line in Fig. 1C crosses the y-axis) but the yield of reads greater than 20KB was clearly higher when using the Blue Pippin, as shown by the red line in Figure 1C being higher than the blue line at a value of 20KB on ...BluePippin Operations Manual 460013 Rev B 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes. My bet is that the nanopore folks have (of course!) done a ton of optimisation on two key things: The DNA cleanup; The library preps; I think that for most DNA extractions cleanups can vary a lot, but something like a Blue Pippin will do an exceptional job if you have access to one and can use it (they won't work for SUPER long DNA fragments which can't move through a gel of course).The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size threshold set by the user. BEVERLY, Mass. — November 11, 2014 — Sage Science, a developer of life science products for improving sample preparation processes in molecular biology applications, today introduced the PippinHT, a high-throughput version of the popular Pippin line of automated DNA size selection instruments for use with next-generation sequencers.A subset of the samples were sent to IBIS (n = 96, including 16 samples from each population) for PstI/MspI library preparation following the procedure of Poland et al. with the following modifications: addition of a size selection step using a Blue Pippin 2% agarose cassette with time settings to elute between 45 and 60 min and a plate barcode ...The Apple Pippin is a defunct open multimedia technology platform, designed by Apple Computer, and marketed as PiPP!N. According to Apple, Pippin was directed at the home market as "an integral part of the consumer audiovisual, stereo, and television environment." ... Sonoran Blue 1996 NA, JP WebViewer with MS Internet Explorer: Non-game, Web ...Check out the latest papers citing automated DNA size selection from Sage Science. Check out our new app note with @Diagenode! Best practices when using #Megaruptor3 w/ #PippinHT or #BluePippin. Includes our new Range+T size selection method & tips on getting tight HMW DNA in the 9-30kb range. #PacBio #HiFisequencing #nanopore #genomics https ... As a new type of fish, GF-L had a high fertilization rate (91.62%) and a hatching rate (82.67%). GF-L presented very beautiful phenotypes, such as twin tails, blue eyes and a white body accompanied by red spots. These phenotypes were very different from any other GF phenotype, showed that the GF-L lineage had great potential in the ornamental ...Cassettes are disposable and pre-cast with agarose and running buffer. Samples are not optically detected.Blotting kits include all the components required to transfer protein, DNA, or RNA samples onto membranes for staining. The materials are grouped specifically for the method and the tissue specimen being implemented, allowing the entire procedure to be completed more rapidly. Using the ...Postal address. Attn. Janneke Weiss. Genome Technology Center (Route 848) Radboud University Medical Center. Geert Grooteplein Zuid 30. 6525 GA, Nijmegen. The Netherlands. [email protected] Tel: +31 (0)24 3617891 Postal address Attn. Janneke Weiss Genome Technology Center (Route 848) Radboud University Medical Center Gee.Safety Data Sheet In accordance with Regulation (EC) No 1272/2008 (REACH) Trade name: BluePippin™ Agarose Gel Cassettes Product No: BDQ3010, BEF2010, BDF2010, BDF1510, PAC20KB, PAC30KB,A subset of the samples were sent to IBIS (n = 96, including 16 samples from each population) for PstI/MspI library preparation following the procedure of Poland et al. with the following modifications: addition of a size selection step using a Blue Pippin 2% agarose cassette with time settings to elute between 45 and 60 min and a plate barcode ...The Blue Pippin and Pippin Prep allow researchers to easily collect from a mixture of DNA fragment sizes, narrow fragment distributions as well as wide ranges of fragments with minimal effort. Maximum DNA fragment size 50 Kb. Up to 5 samples can be ran at once, with no possibility of cross-contamination. Technical Primary Contact:BluePippin Operations Manual 460013 Rev B 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes. The BluePippin produces higher quality DNA, improving downstream analysis. Pre-cast agarose gel cassettes are constructed in a way that eliminates cross contamination. WHY use the BluePippin? (Advantages) The CGRB houses …The BluePippin is an updated version of Sage's Pippin Prep system, to which a pulsed-field power supply has been added. Pulsed field gel electrophoresis allows larger fragments of DNA separated with high resolution and accuracy. Using pre-cast gels, the BluePippin can automatically collect DNA targets up to 50 kilobase pairs, with minimal ...The purified sample was size selected using the Blue Pippin with a dye-free, 0.75% agarose cassette and U1 marker (Sage Science, BUF7510, Mulgrave, Victoria, Australia) and the 0.75% DF Marker U1 high-pass 30-40 kb vs3 run protocol, with a BPstart cut-off of 35,000 bp. After size selection, the samples were purified with AMPure PB beads ...Following adapter ligation, samples were pooled, and these pools were size selected for a range of 430-600 bp using a Blue Pippin (Sage Science). After size selection, samples were PCR-amplified with pool-specific indexed primers, and amplification products were further pooled into a single sample based on molarity calculations from analysis ...Pippin Prep (90bp - 1.5 kb) and Blue Pippin (90bp - 50 kb) are automated DNA sizing tools from Sage Science that offer better reproducibility, accuracy, and yield than manual gels1,2,3,4 while preventing sample cross-contamination. They use a preparative electrophoresis instrument with pre-cast, disposable gel cassettes toIndividuals were then pooled, followed by a size selection step with the Blue Pippin (Sage Science, Beverly, MA) where all loci between 415 and 515 bp were retained. A final PCR step using Phusion Taq polymerase (New England Biolabs) with the following thermocycler conditions was conducted to amplify all loci and attach a 6bp index unique to ...The Genome in a Bottle Consortium, hosted by the National Institute of Standards and Technology (NIST) is creating reference materials and data for human genome sequencing, as well as methods for ...The library was then reduced to fragments in the size range of 250-350 bp using a Blue Pippin (Sage Science, Beverly, MA, United States). Quality and quantity were further verified using TapeStation 2200 (Agilent Technologies). The size-selected, multiplexed samples were run on a single lane of Illumina HiSeq 2500 with 100 bp single-end readsSelection Chart Pippin Prep BluePippin PippinHT Max. DNA input 5 μg 5 μg 1.5 μg Max. Sample Capacity 5 5 24 Pulsed-Field No Yes Yes Run Times (100 bp - 1.5 kb) 50-90 min 50-90 min* 25-45 min* Size Selection Range 100 bp - 1.5 kb 100 bp - 50 kb 100 bp - 1.5 kb High-Pass Size Filtering No Yes Yes Low-Pass Size Filtering Yes Accuracy > 93% ...Sage Science size selecting with bluepippin Size Selecting With Bluepippin, supplied by Sage Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.Blue Pearmain apple. / 3 see reviews. Description; Reviews (10); Tree register (36) ... ©2023 Orange Pippin Ltd. All rights reserved. You may not reproduce any of ...16K views 11 years ago. This video demonstrates how Pippin Prep and BluePippin from Sage Science (www.sagescience.com) perform automated DNA size selection for the …Then the large fragments of DNA were recovered by Blue Pippin automatic nucleic acid fragment recovery system, and repaired. Barcode was added by PCR-free method of EXP-NBD104 kit (Oxford Nanopore Technologies Company). The size of fragments was detected by AATI automatic capillary electrophoresis instrument, and the …The final library was selected using the Blue Pippin protocol for fragments larger than 15 kb. Primer and polymerase were attached using the “Sequel Binding and Internal Ctrl Kit2.1” kit and purification was done using the PacBio “Procedure & Checklist—AMPure ® PB Bead Purication of Polymerase Bound SMRTbell ® …The Blue Pippin also does your size selection, so you don't burn out pores sequencing lots of lit, About Press Copyright Contact us Creators Advertise Developers , He was an eight-time member of the NBA All-Defensiv, When it comes to choosing a web host, there are many options available. One of the m, Sep 9, 2021 · The Blue Pippin was set to select a size range of 15–50 kb. After colle, The ColourTex® stainless steel range is unique whereby, NGS can be roughly divided into the process elements of sample pr, The ColourTex® stainless steel range is unique whereby the colours , Blue Pippin Specs: Electrophoresis voltage: 25V, 100V or 150V constan, In our Iso-Seq analysis, we detected a total of 27,860 transcript, Specifications. Concentration. 0.75%. Product Type. Agarose Gel Cass, Using the Blue-Pippin Size-Selection System (Sage Science Inc., Beverl, 50 years ago, on October 23, 1972, the Broadway cast of a new mu, The staff provide consultation on experimental design, library prepa, Individuals were barcoded with 48 unique barcodes , Page 6 P/N100-377-100-05 Purifying the Large-Scale PCR Products STE, The Genomics Core operates two of these instruments. It combines , Blue Pippin ™ Size Selection System (Sage Science, Beverly, MA) was us.